Robust methods for accurate diagnosis using pan-microbiological oligonucleotide microarrays
نویسندگان
چکیده
منابع مشابه
Mutational analysis using oligonucleotide microarrays.
The development of inexpensive high throughput methods to identify individual DNA sequence differences is important to the future growth of medical genetics. This has become increasingly apparent as epidemiologists, pathologists, and clinical geneticists focus more attention on the molecular basis of complex multifactorial diseases. Such undertakings will rely upon genetic maps based upon newly...
متن کاملDevelopment of a statistically robust quantification method for microorganisms in mixtures using oligonucleotide microarrays.
High-density oligonucleotide arrays can be extremely useful for identifying and quantifying specific targets (i.e., ribosomal RNA of microorganisms) in mixtures. However, current array identification schemes are severely compromised by nonspecific hybridization, resulting in numerous false-positive and false-negative calls, they lack an adequate internal control for assessing the quality of ide...
متن کاملReview article Mutational analysis using oligonucleotide microarrays
The development of inexpensive high throughput methods to identify individual DNA sequence diVerences is important to the future growth of medical genetics. This has become increasingly apparent as epidemiologists, pathologists, and clinical geneticists focus more attention on the molecular basis of complex multifactorial diseases. Such undertakings will rely upon genetic maps based upon newly ...
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Fly stocks Fly culture and crosses were grown on standard fly medium at 25 8C. Oregon R were used as wild-type standard. The BG00650 line was obtained from the Bloomington stock centre. Dredd , Relish, Dif 1 and imd were described elsewhere. The P-element inserted in BG00650 was mobilized by following standard crosses. Mobilization events were screened by PCR using different primer combinations...
متن کاملAn Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays
Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules. Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...
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ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2009
ISSN: 1471-2105
DOI: 10.1186/1471-2105-10-s2-s11